Human chemokine-like factor 1 can improve the poliferation of cardiomyocytes after acute myocardial infarction in rats
- VernacularTitle:人趋化素样因子1促使大鼠心肌梗死后心肌细胞增殖
- Author:
Qianzhu LIU
;
Tao HONG
;
Bo YANG
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Chemotactic factors;
Cell proliferation
- From:
Chinese Journal of Interventional Cardiology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of CKLF1-plasmid transfer on the myocardial repair in rat AMI models.Methods Eighteen male SD rats were randomly divided into 3 groups and in each separate group,the rats were injected intramuscalary with plasmid DNA encoding CKLF1 gene(n=6),emptyplasmid(n=6)and saline(n=6)with in vivo electroporation respectively.Rats were subjected to left coronary artery ligation on the 6th day after gene transfer and were killed on the 21st day.The expressions of BrdU/?-actin,Ki67/?-actin were assessed by immunohistochemistry.Results BrdU-positive cells in CKLF1 group were more than those in the saline group and the empty plasmid group(cells/HP)(33.11?2.10 vs.14.16?1.63 & 18.46?2.77,P0.05).Conclusion Intramuscular injection with in vivo electroporation of CKLF1 may cause an enhanced myocardial proliferation of acute myocardial infarction tissue in experimental rat.
