Effect of S100A8/A9 Protein Complex on F-actin Network in Human Cervical Carcinoma Cell Line,CasKi Cells
- VernacularTitle:钙结合蛋白S100A8/A9对宫颈癌细胞系CasKi细胞F-肌动蛋白的影响
- Author:
Xitao WANG
;
Quanmei SUN
;
Youyi ZHANG
- Publication Type:Journal Article
- Keywords:
Atomic force microscopy;
S100A8/A9 protein complex;
CasKi cells;
Cervical cancer;
F-actin
- From:
Chinese Journal of Minimally Invasive Surgery
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of S100A8/A9 protein complex on the surface morphology and the F-actin network in human cervical carcinoma cell line,CasKi cells.Methods After being cultured with 20 ?g/ml S100A8/A9 protein complex,the cell skeleton of the CasKi cells were observed under a confocal scanning fluorescence microscope by staining the F-actin network.Atomic force microscopy(AFM) was employed to reveal the change of ultrastructure of the cell surface in vivo.ResultsAfter being cultured with the S100A8/A9 protein complex for 24 hours,the F-actin network disorder was revealed.Most of the F-actins distributed peripherally.The OD value of the F-actin decreased significantly from 92.42?5.16 to 57.67?3.70 after been treated with the S100A8/A9(t=5.268,P=0.000).The AFM showed a withdrawing morphology with reduced pseudopodia and destruction of stress fibers. Conclusion S100A8/A9 protein complex can change the ultrastructure of the surface of CasKi cells and its stress fibers by re-distributing of the F-actin in the cells.
