Role of PI3K/AKT signaling pathway in hepatic ischemia-reperfusion-induced lung injury in rats
- VernacularTitle:PI3K/AKT信号通路在全肝缺血再灌注大鼠肺损伤中的作用
- Author:
Chunling JIANG
;
Dong ZHAO
;
Weixing ZHANG
- Publication Type:Journal Article
- Keywords:
PI3K/AKT signaling pathway;
ischemia-reperfusion;
lung injury
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of PI3K/AKT signaling pathway in total hepatic ischemia-reperfusion(I/R)-induced lung injury in rats.Methods This study was divided into 2 sub-projects.(1)36 rats were killed respectively at preischemia and after reperfusion,the lung tissue was then sampled.(2)12 rats were randomly divided into Wortmannin group and model control group. AKT,p-AKT protein expression,apoptotic cells and PCNA protein expression were tested respectively by Western blot,TUNEL and immunohistochemistry analysis.Results(1)Compared with that in preiscemia group,after I/R the apoptotic index (AI)was increased,the p-AKT/AKT ratio and PCNA-positive index showed bidirectional changes,and the histological changes were well identified.(2)p-AKT/AKT ratio showed a positive correlation with PCNA-positive index and a negative correlation with AI.(3)Compared with control group,the p-AKT/AKT ratio and PCNA-positive index were lower,histological changes was more significant and AI was higher in Wortmannin group. Conclusion PI3K/AKT signaling pathway had protective effect on lung injury induced by hepatic I/R,which was potentially mediated by anti-apoptosis and promoting proliferation.
