Optimization of Recombinant E. coli High-density Fermentation for Expressing Human-like CollagenⅡ
- VernacularTitle:重组大肠杆菌高密度发酵生产类人胶原蛋白Ⅱ条件优化
- Author:
Haiyan CHANG
;
Daidi FAN
;
Yane LUO
;
Xiaoxuan MA
;
Yu MI
;
Chenhui ZHU
;
Lei CHI
- Publication Type:Journal Article
- Keywords:
Human-like collagenⅡ, High-density fermentation, Fermentation condition, Induction instant
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To study and optimize the fermentation parameters for expressing human-like collagenⅡduring E. coli high-density fermentation. The effects of pH, temperature, dissolved oxygen and induction instant on the cell growth and human-like collagenⅡproduction were investigated to optimize the fermentation conditions. The results demonstrated that the following conditions were beneficial for cell growth and foreign gene expression, controlling pH in phase induction at 6.8 and initial pH at 6.5, maintaining fermentation temperature and dissolved oxygen concentration was controlled at 34?C and 20% respectively, and implementing induction at the later logarithmic growth phase. Under the optimized condition, the cell density and human-like collagenⅡyield could reach 88.4 g/L and 14.2 g/L, respectively.