Application of Mycoplasma pneumoniae Load Index in Diagnosis of Its Infection in Children
- VernacularTitle:肺炎支原体载量指数在儿童肺炎支原体感染诊断中的应用
- Author:
Jin ZHANG
;
Dakang HU
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumoniae;
Children;
Infection;
Fluorescence quantitative PCR
- From:
Chinese Journal of Nosocomiology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate Mycoplasma pneumoniae load index(MPLI)usefulness in the diagnosis of Mycoplasma pneumoniae(MP) infection in children.METHODS 110 throat swabs were collected with paired sera from the department of pediatry in our hospital from March to July 2008.The former fluorescence quantitative(FQ)-PCR assay was used to analyze throat swabs,microparticle agglutination assay(MAG) and MP-IgM assay for the sera.MP-IgM positive once or twice was used as a gold standard for MP infection,on which sensitivity(SEN),specificity(SPE),positive predictive value(+PV),negative predictive value(-PV) and ROC were calculated.RESULTS According to the gold standard,the values of SEN,SPE,+PV,-PV were 73.1-100.0%for a single MP-IgM,26.9-94.0% for a single MAG,46.2-94.0% for double MAG,26.9-95.2% for FQ-PCR alone and 26.9-98.8% for MPLI,respectively.CONCLUSIONS MAG shows limit in its clinical usage for the detection of MP antibodies.MPLI can diagnose acute MP infection earlier than MP-IgM.
