Atorvastatin Decreased Toll-Like Receptor 4 Expression and Downstream Signals in Human Endothelial Cells
- VernacularTitle:阿托伐他汀下调内皮细胞Toll样受体4及其下游分子的表达
- Author:
Hongyan WANG
;
Peng QU
;
Hua JIANG
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cells;
Atorvastatin;
Lipopolysaccharide;
Toll-like receptor 4;
Nuclear Factor ?B;
Intracellular adhesion molecule-1;
E-selectin
- From:
Chinese Journal of Hypertension
2007;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Background The impact of statins on inflammation are independent of cholesterol-lowering effect.Recent studies showed that Toll-like receptor 4(TLR4),a mediator of innate immune responses,is involved in the initiation and progression of atherosclerosis.Objective To investigate the effects of atorvastatin on LPS-induced TLR4 expression and downstream signals and to explore the molecular mechanisms of anti-inflammation by statins.Methods Human umbilical vein endothelial cells(HUVEC)were pretreated with atorvastatin(1 or 10 ?mol/L)or NF-?B inhibitor CAPE for 30 min,then incubated by purified LPS for 24 hours.TLR4,ICAM-1 and E-selectin mRNA were measured by RT-PCR;the percentage of TLR4 positive cells were detected by flow cytometry.The activation of NF-?B(p65)were detected by Western blot.Results Atorvastatin(1-10 ?mol/L)prevented LPS-induced increases in TLR4,ICAM-1 and E-selectin expression [TLR4 mRNA(1.24?0.21)vs LPS(1.82?0.27),P
