Optimized conditions for growth and in vitro purification of neonatal rat Schwann cells
- VernacularTitle:新生大鼠许旺细胞体外培养纯化的最佳培养条件
- Author:
Bin HE
;
Shiqing LIU
;
Haohuan LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
37 ℃, cell growth became poor, and clone formation decreased. At 39 ℃, cells could not adhere. Under incubator CO2 concentration between 3% and 8%, there was no significant difference in cell clone formation. CONCLUSION: The optimized culture condition of Schwann cells were 7.2 pH, 10% fetal bovine serum, 37 ℃ of temperature and 5% of CO2(v/v) of incubator.
