Effect of Schizandrae Lignanoid on PC12 cell apoptosis induced by H_2O_2
- VernacularTitle:北五味子总木脂素对H_2O_2诱导PC12细胞凋亡的影响
- Author:
Enping JIANG
;
Jinyi WU
;
Jianguang CHEN
- Publication Type:Journal Article
- Keywords:
schizandrae lignanoid;
PC12 cells;
H2O2;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Schizandrae Lignanoid(SCL)on the apoptosis of PC12 cells induced by H2O2 and its relative mechanisms.Methods PC12 cells were divided into four groups: control group,model group,high dose SCL(SCL1) group,and low dose SCL(SCL2) group.Apoptosis of PC12 cells was induced by H2O2.The cell activity was determined by MTT,the cell apoptotic rate was determined by Annexin V-FITC/PI and ??m was detected by flow cytometry.The expressions of bcl-2 and bax were detected by immunohistochemistry.Results Compared with model group,SCL increased the survival rate of PC12 cells(P
