Optimization of bone marrow mononuclear cell apheresis procedure
- VernacularTitle:优化骨髓单个核细胞分离技术的分析
- Author:
Yu JIN
;
Jun ZHOU
;
Bing ZHU
- Publication Type:Journal Article
- Keywords:
Bone marrow procedure, MNC, Apheresis, Clinical treatment
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To optimize the apheresis procedure of mononuclear cells from bone marrow for different clinical treatments. Methods Bone marrow mononuclear cells were separated from bone marrow using COBE Spectra apheresis system. Cell morphology, cell counts, positive expression of CD34, CD133 and CD271 were detected, and the obtaining percent of total nuclear cells and mononuclear cells were calculated. The relationships between cell-morphology and CD34, CD133 and CD271, were also analyzed. Results 1)The positive expression of CD271 was correlated with monocytes before and after apheresis (P
