Cloning and expression of Par6A cDNA
- VernacularTitle:Par6A cDNA的克隆与表达
- Author:
Xiaojun LIU
;
Xingxing KONG
;
Liuluan ZHU
;
Anfang CUI
;
Shaowei JI
;
Yongsheng CHANG
;
Fude FANG
- Publication Type:Journal Article
- Keywords:
Par6A;
cDNA;
rat L6 skeletal muscle cells;
Co-immunoprecipitation
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Cloning and expression of Par6A.Methods Par6A cDNA was amplified from rat L6 skeletal muscle cells by RT-PCR and the cloning and expression vectors of Par6A were constructed.The expression vector was transfected into 293 cells.Furthermore,the function of Par6A was confirmed by Co-immunoprecipitation.Results Par6A cDNA with approximately 1 kb in length was successfully amplified,and the expression vector of pDsRed-Express-N1-Par6A was constructed.The red fluorescene was seen under fluorescent microscope after 293ET cells were transfected for 24 h using the pDsRed-Express-N1-Par6A vector.The expressed Par6A protein can interacte with PKC?.Conclusion We successfully cloned the Par6A cDNA from rat L6 skeletal muscle cells,which provided a reliable method to study the function of Par6A.
