Construction of pGL3-Basic-SREBP-1c-promoter reporter gene vector and detection of its function
- VernacularTitle:人SREBP-1c启动子报告基因的构建和功能验证
- Author:
Xiaojun LIU
;
Xingxing KONG
;
Rui WANG
;
Di SHAO
;
Aijun QIAO
;
Yongsheng CHANG
;
Fude FANG
- Publication Type:Journal Article
- Keywords:
SREBP-1c promoter;
FoxO1;
dual-luciferase reporter assay
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct human SREBP-1c-promoter reporter gene vector and to detect its function.Methods Human blood genome DNA was extracted and pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed.Furthermore,the function of SREBP-1c-promoter was confirmed by dual-luciferase reporter assay.ResultspGL3-Basic-SREBP-1c-promoter reporter gene vector was successfully constructed and the promoter activity was obviously repressed by co-transfection FoxO1.Overexpression FoxO1 inhibited the SREBP-1c protein expression.Conclusion FoxO1 repressed the SREBP-1c protein expression through inhibition the SREBP-1c transcription.
