A new method of inducing mouse primordial germ cells into hepatocytes-like in vitro
- VernacularTitle:诱导小鼠原始生殖细胞向肝细胞样细胞分化的新方法
- Author:
Huafang ZHAO
;
Xiaoyan LI
;
Xiaolin SHI
- Publication Type:Journal Article
- Keywords:
primordial germ cell(PGC);
hepatocyte;
microcapsule;
microenvironment;
inducing differentiation
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new method for inducing the primordial germ cells to differentiate into hepatocyte-like in vitro.Methods The primordial germ cells(PGCs) from the gonadal ridges of the mouse embryos of 13 days postcoitum from Kunming pregnant mice were cultured in vitro.Then embryonic hepatocytes enclosed in microcapsule and liver tissue extract of newborn mice were added into medium to co-culture with PGCs for committed differentiation.Albumin(ALB) and ?-1-antitrypsin(AAT) were assayed by immunocytochemistry.Results The morphology of cells differentiated from PGCs likes star or ovum,the ALB and AAT immune positive expression were detected in those differentiated cells.The ratio of positive cells was above 70% in 2 weeks.Conclusion Microenvironment of embryonic hepatocyte microcapsules and liver tissue extract could effectively induce PGCs to differentiate into hepatocytes.
