VLDL promotes the expression of lipoprotein lipase in human glomerular mesangial cells
- VernacularTitle:VLDL促进脂蛋白脂酶在人肾小球系膜细胞系的表达
- Author:
Jing LI
;
Hang LI
;
Yubing WEN
;
Xuewang LI
- Publication Type:Journal Article
- Keywords:
lipoprotein lipase;
mesangial cells;
very low-density lipoprotein;
glomerulosclerosis
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of lipoprotein lipase(LPL) in human glomerular mesangial cells and the effect of very low-density lipoprotein(VLDL) on the expression of LPL.Methods LPL mRNA expression,protein synthesis and activity were detected in human glomerular mesangial cells by RT-PCR,Western blot and a radio-chemical analysis respectively.Effect of VLDL on the expression of LPL in mesangial cells was detected by Western blot.Results In human glomerular mesangial cells,a 276 bp band,that was specific for human LPL,was identified by RT-PCR,and the same of a 55 kd band,specific for human LPL by western blot.LPL activity of mesangial cells was also detected in the medium after release by heparin.VLDL stimulated LPL protein synthesis in mesangial cells in a time-and dose-dependent manner.Conclusion LPL is expressed by human mesangial cells and it has catalytic activity.Expression of LPL in mesangial cells is regulated by VLDL.
