Combination therapy of Docetaxel with radiation increases apoptosis of human Hela cell line and expressions of TRAIL and its receptors
- VernacularTitle:肿瘤坏死因子相关诱导凋亡配体及其受体在多西紫杉醇对人宫颈癌放疗增敏中的作用
- Author:
Liping TANG
;
Xiaoxing GENG
;
Rong MA
;
Yanmei YANG
- Publication Type:Journal Article
- Keywords:
Docetaxel;
Hela cell;
Apoptosis;
TNF related apoptosisinducing ligand;
TNF related apoptosis-inducing ligand receptor
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Docetaxel as a newly semi-synthesized anti-tumor drug,exhibits significant cyrotoxic activity and enhanced sensitivity for radiotherapy.The aim of this study was to investigate the correlation of the apoptosis of human Hela cell line with the expressions of TRAIL and its receptors induced by the combination therapy of Docetaxel with radiation.Methods: We treated the Hela line by Docetaxel,radiation,and combination therapy of Docetaxel with radiation,respectively,detected the apoptosis of the Hela cells using Hoechst 33342 staining and the TUNEL method,and observed the changes in the mRNA expressions of TRAIL and its receptors TRAIL-R in the three groups by RT-PCR.Results: Compared with the Docetaxel and the radiation groups,the apoptosis of the Hela cells was increased significantly in the combination therapy group,and so were the mRNA expressions of TRAIL and its receptors TRIAL-R1 and TRAIL-R2.Conclusion: The combination therapy of Docetaxel with radiation increased the apoptosis of the Hela cell line,which might be mediated by the up-regulation of mRNA expressions of TRAIL and its receptors TRAIL-R1 and TRAIL-R2.
