Effect of hepatitis B virus X protein on nuclear localization and transcription of ?-catenin in human liver cell
- VernacularTitle:乙肝病毒X蛋白对人肝细胞内?-catenin定位及转录活性的影响
- Author:
Hao DING
;
Liuxing HE
;
Tao FENG
- Publication Type:Journal Article
- Keywords:
Wnt/?-catenin signaling pathway;
HBx protein;
?-catenin;
adenoviruses;
hepatocellular carcinoma
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interaction between HBx protein and ?-catenin for exploring molecular mechanism in HBV-associated hepatocellular carcinoma.Methods Iimmunocytochemistry and Luciferase Assay were used to detect the endocellular location and transcriptional activity of ?-catenin in normal liver cell line L02 infected by Ad-HBx.Results Immunocytochemistry: there was markedly increased expression of ?-catenin in cytoplasm and nucleus.Luciferase Assay: Ad-HBx led to a significant increase of TCF-4-dependent transcription,the TOP count of Ad-GFP and Ad-HBx-GFP was 1.4 fold and 11 fold as compared to the negative control respectively(P
