?-Lactam Genes in Clinical Isolates of Pseudomonas aeruginosa
- VernacularTitle:临床分离多药耐药铜绿假单胞菌?-内酰胺类抗菌药物耐药相关基因研究
- Author:
Peiming LIU
;
Huilin YAO
;
Shihai LU
;
Shuguo QIN
;
Yuanhong XU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Beta-lactamase;
Drug resistance
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the genes associated with the drug-resistance to ?-lactam antibiotics in Pseudomonas aeruginosa(PAE) isolated from clinical patients in Huaibei,Anhui Province.METHODS Antibiotic sensitivity test was performed by VITEK-AMS.The three-dimensional method was taken to differentiate various beta-lactamases.The ?-lactamases and the outer membrane protein D2(oprD2) genes were detected by polymerase chain reaction(PCR) in 36 PAE strains.RESULTS In 36 PAE strains,the positive rates of genes of TEM,DHA,CTX-Ge and CARB were 77.8%,69.2%,27.8% and 25.0%,respectively,that of the OXA-1,OXA-10,VIM-2 and IMP-1 were 16.7%,16.7%,13.9% and 2.8%,respectively.The rate of lacking oprD2 gene was 58.3%,and none possessed VEB,SHV,GES,PER,GIM and SPM-2 genes.CONCLUSIONS P.aeruginosa carries various beta-lactamase genes in clinical patients of Huaibei,and the deletion ratio of oprD2 gene is higher.
