The expression of neutrophils FCγRⅡ/Ⅲ modulated by anti-neutrophil cytoplasmic antibodies and its clinical value
- VernacularTitle:抗中性粒细胞胞质抗体对中性粒细胞表面FcγⅡ/Ⅲ受体表达的影响及其意义
- Author:
Xiangling WANG
;
Nan CHEN
;
Haijin YU
;
Hong REN
;
Weiming WANG
;
Liyan NI
- Publication Type:Journal Article
- Keywords:
Antibodies,antineutrophil cytoplasmic;
Receptors,IgG;
Vasculitis
- From:
Chinese Journal of Rheumatology
2008;12(7):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The interaction between anti-neutrophil cytoplasmic antibaties (ANCA) and receptors at the Fc portion of immunoglobulins (FeγR) is central in the pathogenisis of primary systemic small vasculitis. The aim of this study is to investigate the role and clinical value of ANCA on the expression of neutrophils FCγRⅡ/Ⅲ (CD32/CD16). Methods ANCA IgG was prepared from the sera of patients with active We-gener's granulomatosis (WG) and microscopic polyangiitis (MPA). Neutrophils were isolated from the blood of healthy volunteers. The expression of CD32/CD16 on neutrophils was assessed by flow cytometry after stimulated by ANCA for 1 hour. We compared the expression of CD32/CD16 between 18 primary systemic small vaseulitis (PSV) patients and 35 healthy volunteers. Furthermore, the correlation was also be analyzed between the expression of CD32/CD16 and Birmingham vasculitis activity score (BVAS). Results The expression of CD16 was significandy elevated by ANCA (Mnx 67±23 vs 54±21, P<0.01 ). The expression of CD16 was higher in patients than in healthy volunteers (Mnx 62±12 vs 53±10, P<0.01), which was in correlation with BVAS (r=0.728 86, P<0.01). But no such correlation was found for CD32 . Conclusion ANCA may play a role in the pathogenesis of PSV by modulating the expression of the FCγR. Monitoring the expression of CD16 on neutrophils is helpful for the evaluation of PSV activity.
