Characterization of Key Regulatory Elements of LCRG1 Promoter
- VernacularTitle:LCRG1基因启动子关键顺式调节元件的鉴定
- Author:
Hailong XIE
;
Zhuchu CHEN
;
Jinhua LI
;
Longwu ZENG
;
Guihuang TAN
- Publication Type:Journal Article
- Keywords:
LCRG1 gene,laryngeal carcinoma,minimal promoter,transcriptional regulation,cis-elements,transcription factor Sp1
- From:
Progress in Biochemistry and Biophysics
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
LCRG1(laryngeal carcinoma related gene1,LCRG1),a new candidate tumor suppressor gene of laryngeal carcinoma.However,it is known little about the possible regulatory mechanisms of LCRG1 gene expression.Restriction endonuclease digestion was used to obtain a set of the 5',or 3'deletion mutants from the region(-169～+127) of the LCRG1 gene.It has been found that the minimal promoter of the LCRG1 gene is mapped at the region from-169～-57.Linker scanning mutational analysis in the region(-169～+127) of the LCRG1 gene was used to identify the crucial cis-elements within the promoter region,The key cis-elements are within the region from-137～-122.SP1,E2F1/DP1,EKLF and ZF9 transcription factor binding site sites were predicted in the region by bioinformatics analysis.Co-transfection with each of a panel of the expression plasmids of the known transcription factors with the relevant reporter construct indicates Sp1 is potent transcription factor for enhancement of the promoter activity,SP1 can also up-regulate the endogenous expression of LCRG1 gene.Electrophoretic mobility shift assay(EMSA) was applied to verify that the key cis-elements of LCRG1 gene exist sequence of Sp1 binding sites.The findings,which showed that the key cis-elements within the region from 137～-122 play an important role in expression of the LCRG1 gene,provide a novel evidence for further study of the function of LCRG1 gene.
