Aptamer 6-10 of DNA polymerase can increase the sensitivity of quantitative PCR
- VernacularTitle:DNA聚合酶适配子6-10提高定量PCR的特异性
- Author:
Fengtao LIU
;
Lihui CAI
;
Yuanfan MA
- Publication Type:Journal Article
- Keywords:
aptamer;
DNA polymerase;
quantitative PCR;
sensitivity
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To increase the sensitivity of quantitative PCR using DNA polymerase in combination with aptamer 6-10.Methods The quantitative PCR was performed using DNA polymerase mixed with aptamer 6-10 or specific antibody.The low detection limit of each modified method was compared with that of the conventional method.Results The quantitative PCR using aptamer 6-10(200 nmol/L) or specific antibody increased the low detection limit of human death receptor 5(DR5) plasmid from 105 copies/?l to 103 copies/?l.Melting curves showed that each method had minimal nonspecific amplification when the concentration of DR5 plasmid was 105 copies/?l;however,when the concentration of DR5 plasmid was 103 copies/?l,the conventional method had nonspecific amplification,whereas the method using aptamer 6-10 had minimal nonspecific amplification.Conclusions Aptamer 6-10 of DNA polymerase can increase the sensitivity of quantitative PCR.
