Effects of platelet-rich plasma on proliferative and osteogenetic activity of skeletal muscle stem cells in vitro
- VernacularTitle:富血小板血浆对体外培养骨骼肌干细胞增殖及成骨活性的作用
- Author:
Liancheng SHAN
;
Gang WANG
;
Changqing ZHANG
;
Bingfang ZENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies has confirmed that platelet-rich plasma(PRP) can affect the proliferation and differentiation of bone marrow mesenchymal stem cells and adipose derived stem cells. OBJECTIVE:To observe the effects of PRP on the proliferative and osteogenetic activity of skeletal muscle stem cells(SMSCs) in vitro,in addition,to elucidate the potential mechanism by which PRP affects SMSCs. DESIGN,TIME AND SETTING:A randomized controlled animal experiments was completed in the Center Laboratory of the Sixth People's Hospital Affiliated to Shanghai Jiao Tong University from June to October in 2008. MATERIALS:Nine New Zealand,ordinary grade rabbits,weight of 2.5-3.0 kg,1 year old,irrespective of gender. METHODS:The right soleus was dissected and cultured for SMSCs. PRP was prepared with central artery of rabbits’ ears. The cells were randomly divided into the experimental group and the control group. In the experimental group,the SMSCs were interfused with PRP(special culture media including 12.5% PRP) . There was no intervention in the control group. MAIN OUTCOME MEASURES:①Morphological observation. ②The proliferative ability of the cells was detected with MTT. ③ The osteogenetic ability was measured with alkaline phosphatase(AKP) staining,alizarin red staining and osteocalcin immunofluorescence assay. RESULTS:MTT method showed that the proliferative activity of the experimental group was obviously stronger than that of the control group(P
