Comparison of local and systemic application of ibandronate on fracture healing in rabbits
- VernacularTitle:全身与桡骨骨折局部应用伊班膦酸钠对骨折愈合影响的比较
- Author:
Lei ZHANG
;
Chen WANG
;
Xuejun ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Ibandronate is the typical representative of bisphosphonates drugs,which prevent bone absorption. OBJECTIVE:To verify the effect of Ibandronate on fracture healing after local application. DESIGN,TIME AND SETTING:A randomized control experiment was accomplished in the Medicine Center Laboratory of Southeast University in October,2008. MATERIALS:Thirty-six healthy New Zealand rabbits,aged 6-8 month,weighting 2.1-2.6 kg. Ibandronate injection(1 mL:1 mg) was supplied by Biomedical Engineering Centre of Hebei Medical University. METHODS:Thirty-six healthy New Zealand rabbits were randomly divided into the control,systemic application and the local application groups,with 12 rabbits in each group. All the rabbits were prepared fracture healing models. Rabbits in control group were given no more treatment after model preparation. Animals in the systemic application control group was given 0.1 mg Ibandronate injection through vein or locally. The state of fracture healing were examined and then scored by X-ray at week 2,4,6 and 8 after model preparation. At weeks 1,2,3 and 4,the level of serum tartrate-resistant acid phosphatase 5b(TRAP-5b) were assayed with ELISA. At weeks 2,4,6 and 8,three rabbits were selected from each group randomly and were killed by aeroembolism respectively. Their calluses were intercepted from fracture sites as the research objects. Pathological changes were observed in callus samples and immunohistochemistry stain was performed to examine type I collagen expression in tissues of callus. MAIN OUTCOME MEASURES:①Fracture healing scores. ②The level changes of TRAP. ③The percentage of bone trabecula area. ④Osteoblast index. ⑤Absorbance value. RESULTS:All rabbits were involved in the result analysis. The systemic application group and local application group obtained higher scores than in the control group at weeks 2(P
