Genetic Marker of Integron,Transposon in Escherichia coli Isolated from Elderly Patient
- VernacularTitle:老年患者感染大肠埃希菌分离株整合子、转座子遗传标记研究
- Author:
Faxiang JIN
;
Jianping ZHONG
;
Jialiang TANG
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Integron;
Transposon;
Gene mark;
Polymerase chain reaction
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the existance of class Ⅰ gene integron and transposon(merA and tnpA) gene marker in Escherichia coli isolated from the elderly patients.METHODS Antibiotic sensitivity was performed by microdilution test.Integron gene IntⅠ1 and transposon marker genes were detected by polymerase chain reaction(PCR).The PCR products were purified and sequenced.RESULTS E.coli resistant to amikacin accounted for 60%,to sulfamethoxazole 95%,and to third-generation cephalosporin 100%(all ESBLs-producing strains).The resistance was related closely to the class Ⅰ integron.The positive rate of integron intⅠ1 was 80%(16/20),whereas the positive rate of gene merA was only 10%(2/20).Gene tnpA was not tested out.CONCLUSIONS The drug resistance of E.coli has close relation with transmission of integron and transposon.The transposons of Tn21/Tn501 are all firstly reported in China.
