Distribution of mecA in Staphylococcus aureus and Its Effect on Antibiotics-resistance
- VernacularTitle:mecA基因在金黄色葡萄球菌中的分布及对耐药性的影响
- Author:
Qingzeng CHEN
;
Bing LUO
;
Yingjuan SUN
;
Weili HUANG
;
Ping QIN
- Publication Type:Journal Article
- Keywords:
mecA;
Staphylococcus aureus;
MRSA;
Antibiotics-resistance
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand mecA gene distribution in Staphylococcus aureus and its role in antibiotics-resistance.METHODS In this study,a total of 47 S.aureus strains were isolated from hospitalized patients.Agar disk diffusion test was conducted to determine the resistance of S.aureus to antibiotics.The DNA of these strains were extracted and purified.The mecA gene was tested by PCR and the relation between the mecA gene and antibiotics-resistance was analyzed.RESULTS Of 47 strains,33(70.2%) were MRSA.Of 33 MRSA,only 3 strains were susceptible to glycopeptides antibiotics.Only 2 strains(14.3%) of 14 MSSA were susceptible to all of the 12 antibotics.The results of PCR revealed that 32 out of 33 MRSA(97.0%) carried mecA in their genome.One strain was mecA gene negative.Among 14 MSSA,3(21.4%)strains carried mecA gene.CONCLUSIONS The isolation rate of MRSA in S.aureus is high.The resistance to antibiotics of MRSA is popular Glycopeptides antibiotics.Most of MRSA carry mecA gene,which plays an important role in antibiotics-resistance.Fewer MSSA carry mecA gene.
