Combined Detection of Rifampin-and Isoniazid-resistant Mycobacterium tuberculosis by DNA Chip and Its Clinical Application
- VernacularTitle:DNA芯片联合检测耐利福平与异烟肼结核分枝杆菌及其临床应用
- Author:
Ganhu YE
;
Baoyuan MAI
;
Fang LIU
;
Xingen OUYANG
;
Jianqing LI
;
Shengyun LIAO
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Genes rpoB,katG and inhA;
Rifampin;
Isoniazid;
DNA chip
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a new DNA chip for rapid detection of rpoB,katG and inhA gene mutation in rifampin and/or isoniazid-resistant Mycobacterium tuberculosis isolated from clinical laboratorial specimen.METHODS We designed 16 oligonucleotide probes specific for detection of the mutant sequences in genes rpoB,katG and inhA.RESULTS Mutations were found in 26 strains(100%) of 26 randomly selected rifampin-resistant M.tuberculosis and 24 strains(80%) of 30 randomly selected isoniazid-resistant M.tuberculosis by DNA chip.CONCLUSIONS DNA chip technology has high sensitivity and specificity in detection of rifampin-resistant M.tuberculosis and may be applied in clinical diagnosis.
