Chemokine Fractalkine Induced Monocytes Adhesion on Human Umbilical Vascular Endothelial Cells
- VernacularTitle:趋化因子分形素Fractalkine介导的单核细胞黏附
- Author:
Yilun TIAN
;
Deqian JIANG
- Publication Type:Journal Article
- Keywords:
Chemokine;
Fractalkine;
Human umbilical vascular endothelial cell;
Monocyte THP-1;
Adhesion;
Interleukin-18;
Atherosclerosis
- From:
Chinese Journal of Hypertension
2007;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Background Fractalkine(FKN) is a novel chemokine that mediate the adhesion of monocyte to vascular endothelial cell,involving in the development of atherosclerosis(AS).Objective To investigate the effect of interleukin-18(IL-18) on the fractalkine expression in human umbilical vascular endothelial cells(HUVEC),and the role of fractalkine in the monocytes THP-1 adhesion on HUVEC.Methods Fractalkine expression in HUVEC was determined by reverse transcription-polymerase chain reaction(RT-PCR),and the adhesion of THP-1 to HUVEC was assessed by Flow Chamber.Results Incubation of HUVEC with IL-18 upregulated FKN mRNA expression in dose-dependent manner.In the concentration of IL-18 ranged 0,25,50,100 ?g/L,the FKN mRNA was increased from(0.10?0.01),(0.49?0.04),(0.63?0.09) to(0.85?0.07)(P trend
