Expression of ATPase F1? in human colorectal cancer tissues and cell line and its clinical significance
- VernacularTitle:ATPase F1?在人结直肠癌组织和细胞株中的表达及其意义
- Author:
Minyu LI
;
Haimo ZHU
;
Junping ZHOU
;
Guoliang LOU
- Publication Type:Journal Article
- Keywords:
ATPase F1?;
colorectal neoplasms;
immunohistochemistry;
immunofluorescence;
CCK-8
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of ATPase F1? in colorectal cancer(CRC) tissues and in LoVo cells,and to discuss its clinical significance.Methods:Expression of ATPase F1? protein in 44 CRC specimens and their adjacent normal tissues(August 2007 to December 2007,Changhai Hospital) and ATPase F1? mRNA in 8 colorectal cancer tissues and their adjacent normal tissues were examined by immunohistochemistry EnVision assay and RT-PCR,respectively.Expression of ATPase F1? on the cell surface of LoVo cells was observed by immunofluorescence.The inhibitory effect of anti-ATPase F1? antibody on the proliferation of LoVo cells was evaluated by CCK-8 assay.Results:Expression of ATPase F1? in the 44 CRCs were significantly higher than those in the adjacent normal tissues as detected by immunohistochemistry(P0.05).Expression of ATPase F1? was observed on the cell surface of LoVo cells,and anti-ATPase F1? antibody significantly inhibited the proliferation of LoVo cells(P
