Construction and identification of a screening vector using enhanced green fluorescent protein(EGFP) as an indicator
- VernacularTitle:利用增强型绿色荧光蛋白基因作筛选标记克隆载体的构建及鉴定
- Author:
Jinbao TANG
;
Yong CHEN
;
Shujuan LIANG
;
Huanqin WANG
;
Qingchun LI
- Publication Type:Journal Article
- Keywords:
enhanced green fluorescent protein (EGFP);
E.coli;
positive clone;
screening vector
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify the screening vector pUC18-EGFP,using EGFP as an indicator. Methods The EGFP gene was prepared by PCR and cloned into pUC18 resulting the vector pUC18-EGFP. Then DNA fragment was inserted into the MCS of pUC18-EGFP to test its practicability based on green fluorescence. Results The pUC18-EGFP was confirmed correctly by restriction enzyme analyses. The pUC18-EGFP was used to select recombinants. The green strains on the plate were confirmed by restriction enzyme and DNA analyses,which were E.coli harboring recombinants. Conclusion The screening vector PUCl8-EGFP was constructed successfully. Thus,we can select the positive clones on plates based on the green fluorescence of EGFP.
