Study of the effect on differentiation of bone marrow mesenchymal stem cells induced by Ganglioside into neural cells
- VernacularTitle:神经节苷脂诱导骨髓间充质干细胞分化为神经细胞的作用的研究
- Author:
Yan LIU
;
Yingdong ZHANG
- Publication Type:Journal Article
- Keywords:
Ganglioside;
bone marrow mesenchymal stem cells;
differentiation;
neurotrophic factors
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect on the differentiatiation of bone marrow mesenchymal stem cells(BMSCs) induced by Ganglioside(GM1) into neural cells.Methods Rat BMSCs were isolated on the basis of its ability to adhere to the culture plate,passaged three times,and finally added GM1 to induce its differentiation.The morphologic changes of BMSCs were observed under phase-contrast microscopy,then neuron specific enolase(NSE) and glial fibrillary acidic protein(GFAP) expression were determined by immunocytochemistry way.Never growth factor(NGF) mRNA and brain-derived neurotrophic factor(BDNF) mRNA expression were detected with RT-PCR and then compared with those of FBS control group and blank control group.Results In GM1 induction group,BMSCs appeared round-shaped,with some dendrite and axon interlaced.(29.47%?3.26)% BMSCs showed positive staining to NSE,while(2.32?0.18)% were positive to GFAP.(6.97?0.56)% and(10.6?0.75)% BMSCs were positive to NSE in FBS control group and blank control group respectively,while(1.41?0.35)% and(1.21?0.35)% BMSCs were positive to GFAP in FBS group and blank control group respectively.NGF mRNA and BDNF mRNA levels in GM1 induction group were significant higher than those in the FBS group(all P
