Regulatory effect of triptolide on angiogenesis in synovium of collagen-induced arthritis rats
- VernacularTitle:雷公藤甲素对胶原诱导关节炎大鼠滑膜血管新生的调节作用
- Author:
Zhe CHEN
;
Yonghong HU
;
Yu WANG
;
Shenghao TU
;
Peilin LIU
;
Hong BA
;
Xiaojuan GUO
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factors;
Fibroblast growth factor 2;
Endostatins;
Tiptolide
- From:
Chinese Journal of Rheumatology
2008;12(4):254-257,插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory effect of triptolide(TP)on the angiogenesis of coll-agen-induced arthritis(CIA) rats.The effect of TP on arthritis is also explored.Methods After the model of CIA was established,the articular volume was measured and the synovium was examined with regular HE stainand the inflammation and pathological changes were evaluated.In addition,the vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF)and endostatin protein expressions in synovium and serum were tested.The micro-vessel density (MVD) of synovium was also measured by caulating CD34 level.Results The expressions of VEGF,bFGF and MVD in CIA rats'synovium and serum were evidently higher than the control group(X2=65.3,31.6,q=9.2,P＜O.01,respectively),while the expression of endostatin showed no statistical difference with controls (X2=0.8,P＞0.05).After treated with TP,the expressions of VEGF,bFGFand MVD decreased markedly(X2=19.7,6.0,q=6.5,P＜O.01,respectively),but the pmtein expression of endostatin significantly increased (X2=3.9,P＜O.05).However,only the expression of endostatin increased significantly after treated with MTX (X2=17.9,P＜0.01).Conclusion Imbalance in growth factors prnduction may play an important role in the process of arthritis development.Re-establishing the balance of growth factors maybe one of the mechanisms of TP in the treatment of arthritis.
