Proliferative response of peripheral blood mononuclear cells and its depressant effect on small cell lung cancer cells in patients with paraneoplastic neurological syndrome
- VernacularTitle:副肿瘤性神经系统综合征患者外周血单个核细胞的增殖反应及其对小细胞肺癌细胞株的抑制作用
- Author:
Liang YIN
;
Lina ZHANG
;
Baiqing LI
- Publication Type:Journal Article
- Keywords:
small cell lung cancer,cell line NCI-H446;
paraneoplastic neurological syndromes;
culture in vitro;
proliferation
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferative response of peripheral blood mononuclear cells(PBMC) and its depressant effect on small cell lung cancer(SCLC)cells(H446) in patients with paraneoplastic neurological syndrome(PNS).Methods PBMC of 7 patients with PNS and 6 patients with SCLC were cultured with interleukin(IL)-2 in vitro,then cultured separately or mixed with H446 respectively.The proliferation index(PI,the ratio of cellular score which had proliferated and that had not proliferated) of H446,PBMC,CD4+,CD8+ T cell and the ratio of CD4+,CD8+,CD4+/CD8+ were analyzed with flow cytometry and compared with normal control group.Results Compared with cultured separately,the PI of H446,PBMC,CD4+,CD8+T cell in PBMC of PNS and SCLC groups cultured with H446 were not significantly different.Stimulated by IL-2,the ratio of CD4+ T cell in PNS patients [(76.54 ? 3.96)%]was higher than that in normal control group[(51.75 ? 17.3)%](P
