Study of the bone marrow mononuclear cell apheresis procedure and clinical applications
- VernacularTitle:骨髓单个核细胞分离技术研究及临床应用
- Author:
Jun ZHOU
;
Lixia FAN
;
Haiyan DU
- Publication Type:Journal Article
- Keywords:
Bone marrow procedure;
MNC;
Apheresis
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apheresis procedure of collecting mononuclear cells from bone marrow for clinical treatment.Methods Bone marrow mononuclear cells were separated from bone marrow using COBE Spectra apheresis system.Cell morphology,cell counts,positive expression of CD34+,CD133+,CD271+ were detected,and the recovery rate of nucleated cell and mononuclear cell were also calculated.And the main parameters of the procedure with upper efficiency were set via data analysis.Results The efficiency of collection line with colourimetric card of 3% was higher than that of 5% and of 7%.The recovery rate of mononuclear cell is 75% when the cycle times ranged from 7 to 9.The positive expression of CD34+,CD133+ and CD271+ were 1.17%,0.63%,and 0.53%,respectively.Conclution This mononuclear apheresis procedure is safety,pollution-free and efficient,and remaming the bone marrow could be retransfused to the donor.
