The diagnostic Value of Three Methods Among autoimmune diseases
10.3760/cma.j.issn.1673-4130.2010.06.009
- VernacularTitle:3种检测方法在诊断自身免疫性疾病中的价值
- Author:
Zuoxin MA
;
Na LIAN
;
Li ZHENG
- Publication Type:Journal Article
- Keywords:
antibodies,antinuclear;
antigens;
autoimmune diseases
- From:
International Journal of Laboratory Medicine
2010;31(6):540-541,543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study more significant detective combination by comparing three methods of antinuclear antibody,extractable nuclear antigen and antinuclear antibodies 3.Methods Three methods were adopted as follows:detecting antinuclear antibody with HEP-2 and prime hepar as antigen media,determining extractable nuclear antigen by EUROASSAY including nRNP/Sm、Sm、SSA、SSB、Scl-70 and Jo-1,using crithidia iuciliae as antigen,to detect ds-DNA antibody with indirect immunofluorescence assay,and to study 15 autoantibodies by EUROLINE.Results To compare positive rates of three dilution(1:10,1:80,1:100)in ANA detection,there existed significant difference(P<0.01)between two dilution of 1:10 and 1:80 only,.In the detection of multiple nuclear types(cytoplasmic pattern,centromere pattern and homegeneous pattern etc),the positive rate of antinucleatr antibodies 3 was higer than that of ENA.IIF was prior to the EUROLINE in the detection of ds-DNA.Conclusion During detecting ANA with IIF,the dilution of 1:80 has advantages in raising positive rate,and ANA3 of 15 antigens provides facilities for clinical diagnosis.
