Effect of Different Types of Pneumoperitoneum on the Ultrastructure of the Extraperitoneal Muscular Tissues in Rat
- VernacularTitle:2种不同工作原理气腹机形成CO_2气腹对大鼠腹膜浅方肌组织超微结构的影响
- Author:
Haifang LIU
;
Xu CHEN
;
Runhan HUANG
- Publication Type:Journal Article
- Keywords:
CO2 pneumoperitoneum;
Peritoneum;
Myocyte;
Transmission electron microscope (TEM)
- From:Chinese Journal of Minimally Invasive Surgery
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of different types of pneumoperitoneum on the ultrastructure of the extraperitoneal muscular tissues in rats. Methods By using pulse-mode or constant-pressure mode insufflators,we established CO2 pneumoperitoneum in 80 rats (40 for each) with the pressure set at 15 mmHg. At 60,90,120 and 180 minutes after the insufflation,the muscular tissues below the peritoneum of the rats (10 rats at each time point) were obtained to observe the ultrastructural change under a transmission electron microscope (TEM). Ten rats that received anesthesia only were set as a control. Results In both the groups,vacuolar degeneration was found in the myocytes below the peritoneum after 60 minutes of pneumoperitoneum,and became more and more marked with time. Moreover,the phenomenon was more significant in the pulse-mode insufflator group,in which,we observed widened gaps and vacuoles among the myocytes at 180 minutes after the insufflation,while in the other group,such changes were not apparently detected. Conclusions CO2 pneumoperitoneum may injure the muscular tissues below the peritoneum in rats. Constant-pressure mode insufflator is helpful to decrease such an injury.
