Rapid Diagnosis of Legionella pneumophial Pneumonia Used Polymerase Chain Reaction
- VernacularTitle:聚合酶链反应在嗜肺军团菌性肺炎诊断中的应用
- Author:
Jun WANG
;
Lianyou ZHENG
;
Haitao PENG
;
Zhaohui HU
;
Yuanli LIU
;
Qingyi ZHU
- Publication Type:Journal Article
- Keywords:
Legionella pneumophila;
Atypical pneumonia;
Polymerase chain reaction(PCR)
- From:
Chinese Journal of Nosocomiology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To prove the diagnosis value for Legionnaires pneumophial pneumonia using polymerase chain reaction. METHODS L. pneumophial-DNA (LPN-DNA) from 47 spuum and 6 bronchoalveolar lavage fluid samples collected from 53 patients with atypical pneumonia was detected by PCR. RESULTS The positive rate of LPN-DNA in 53 patients with atypical pneumonia was 9.4%, while the positive rate of sputum and bronchoalveolar lavage fluid samples was 6.4%and 33.3%, respectively. CONCLUSIONS LPN-DNA detected by PCR for early diagnosis of atypical pneumonia has favorable clinical application.