Regulation of LCRG1 promoter transcription activity by Sp1 and Egr-1
- VernacularTitle:Sp1和Egr-1对LCRG1基因启动子转录活性的调节研究
- Author:
Hailong XIE
;
Longwu ZENG
;
Xiaojun ZHOU
- Publication Type:Journal Article
- Keywords:
LCRG1;
Laryngeal carcinoma;
Promoter
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The regulatory mechanisms of LCRG1 gene expression is undear,this study was to investigate the transcription regulation of the promoter of human LCRG1 gene by transcription factors Sp1 and Egr-1.Methods:Analysis of putative binding sites of transcription factors in the promoter of human LCRG1 gene by online program MatInspector,Co-transfection with the expression plasmids of the known transcription factors such as Sp1、wtEgr-1、mtEgr-1with the LCRG1 reporter construct analyze potent transcription factor for enhancement of the promoter activity.Results:SP1 and Egr-1 transcription factor binding site sites were predicted in the region by bioinformatics analysis,mtEgr-1 can enhance the promoter activity.Conclusion:mtEgr-1 may be involved in the regulatory mechanisms of LCRG1 gene expression
