In situ Fabrication and Application of Protein Microarray With Cell-free System
- VernacularTitle:无细胞系统原位制备蛋白质芯片及其应用
- Author:
Jie ZHANG
;
Qiongming LIU
;
Danke XU
;
Fuchu HE
- Publication Type:Journal Article
- Keywords:
protein microarray, protein expression with cell-free system, in vitro protein expression, in situ fabrication of protein arrays, cell-free system
- From:
Progress in Biochemistry and Biophysics
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Protein is one of major bio-functional performers. As one of several crucial proteomic research approaches, protein microarray has these following advantages: high-throughput, high sensitivity, quick detection and so on. Meanwhile, there are some critical factors that are important to the further development of protein microarray technology, for example, how to express and purify proteins for the research of protein microarray, how to immobilize proteins onto the substrate and keep the bio-function of proteins immobilized. Nano-biotechnology and cell-free expression system have been used to fabricate protein microarray by the way of immobilizing target genes onto the substrate and directly expressing corresponding proteins, which provides a new strategy to fabricate more complicated microarray. The stragegy and its progress were summarized———fabrication of protein microarray based on DNA, including immobilization of target genes, cell-free expression to proteins, immobilization of renascence proteins, advantages and drawbacks of the methods of protein chip fabrication etc.
