Desert Hedgehog induces the differentiation of neural progenitor cells from embryonic mesencephalon of rat into dopaminergic neurons
- VernacularTitle:Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元
- Author:
Caixia YANG
;
Huanying ZHAO
;
Chunli ZHAO
;
Jing LIU
;
Qunyuan XU
;
Fulu GAO
- Publication Type:Journal Article
- Keywords:
Desert Hedgehog;
neural progenitor cell;
dopaminergic neuron;
differentiation;
rat
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Desert Hedgehog on direct differentiation of neural progenitor cells(NPCs) cultured from embryonic mesencephalon in the rats.Methods We infected DHH into COS7,NIH/3T3 and 9L cells,and detected the expression of DHH in the cells with immunofluorescence,real-time PCR and Western blot.All of the three cells were co-cultured with NPCs isolated from ventral mesencephalon in embryonic SD rats(E13.5) respectively.Immunoreactivities of tyrosine hydroxylase(TH) was detected by immunocytochemistry after 10 days.Results The expression of DHH in COS7,NIH/3T3 and 9L cells was remarkably detected,but few TH-positive cells were found in the three co-cultral systems at the 10th day.Conclusion The protein derived from DHH itself does not show any inductive effect on the differentiation of NPCs to the dopaminergic neurons in vitro.
