Effect of FADDDEL-GFP modification in murine islet cell transplantion
- VernacularTitle:FADDDEL-GFP基因修饰在小鼠胰岛细胞移植中的作用
- Author:
Ping HU
;
Sha WU
;
Xiaolan LI
;
Jing WEI
- Publication Type:Journal Article
- Keywords:
FADD;
type 1 diabetes;
transfection;
transplantation
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of fusion protein FADDDEL-GFP in the treatment of type 1 diabetes by murine islet cell transplantion.Methods After transfecting the recombinate FADDDEL-GFP into mouse insulinoma cells NIT-1,insulin level secreted by the cells was examined.Type 1 diabetes was induced by STZ as animal model.NIT-fg cells,the NIT-1 cells modified by FADDDEL-GFP,were transplanted into the diabetic mice.Then the effects of islet cell transplantation on diabetic mice were checked.Results GFP was expressed in NIT-1 cells transfected with FADDDEL-GFP.Diabetic mice reached normoglycemia and prolonged the life span,which was obviously different from the control group.Conclusion FADDDEL-GFP may enhance the capability of resisting allogeneic transplantation rejection.
