Antisense oligonecleotides of CT120 inhibit the growth of lung adenocarcinoma cell line A549
- VernacularTitle:CT120反义寡核苷酸抑制肺腺癌细胞A549的生长
- Author:
Zunling LI
;
Shuhong SHAO
;
Shuyang XIE
;
Fei JIAO
;
Shouseng SHI
;
Xueyan YU
- Publication Type:Journal Article
- Keywords:
CT120;
antisense oligonecleotides;
lung cancer
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the relationship between CT120,a novel human plasma membrane-associated gene,and the proliferation of lung adenocarcinoma cell line A549.Methods Expression vector(pcDNA3.1)containing antisense oligonucleotides of CT120 was constructed and transfected into the adenocarcinoma cell line A549.RT-PCR and Western blot detected the expression of CT120.Meantime,flow cytometry and soft agarose colony formation were applied for cell proliferation,and P53,CyclinD1 and CDK4 were detected by RT-PCR.ResultspcDNA3.1 containing antisense oligonucleotides of CT120 was successfully constructed and inhibited the expression of CT120 effectively by RT-PCR and Western blot.The expression of P53 was up-regulated and the expression of CyclinD1 and CDK4 were down-regulated.Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a potential drug target for treatment of lung cancer.
