Otinulation of osteolytic cytokine expression by micrometer-diameter wear particles
- VernacularTitle:微米级磨损微粒刺激溶骨性细胞因子表达
- Author:
Ming CHEN
;
Qirong DONG
;
Yu LEI
- Publication Type:Journal Article
- Keywords:
wear particles;
animal model;
osteolytic cytokines;
expression
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact osteolytic cytokines of expression induced by micrometer-diameter wear particles(Ti-6Al-4V and UHMWPE).Methods Filtration air was subcutaneously injected into rats'back 6 times(3 mL q?d).After a week,wear particles suspension(group A: Ti-6Al-4V,group B: UHMWPE) or physiological saline(group C) was injected into air pouch tissues.After 14 days,pouch tissues were obtained from killed rats,and were weighted,wax embedded and stained with hematoxylin and eosin, observed under microscope.AKP of serium with Automated Biochemical Analyzer,IL-6 and TNF-? expression with immunohistochemical method,and mRNA expression of extracellular matrix metalloproteinase inducer(EMMPRIN) with real-time Polymerase Chain Reaction method were detected.Results Air pouch tissues were similar to limiting membrane of periprothesis tissue in the cases of aseptic loosening.As to pouch tissue weight,there was a significant increase in group B than in group C(P
