Research on Clostridium Difficile Infection in Clinic Patients Feces Specimen
- VernacularTitle:临床患者粪便标本中艰难梭菌感染状况研究
- Author:
Ying CHENG
;
Jinxing LU
;
Shengkai YAN
;
Jing WANG
;
Jie LI
;
Yingchun LIU
- Publication Type:Journal Article
- Keywords:
Clostridium difficile;
Clostridium difficile-associated diarrhea;
Anaerobic culture
- From:
Chinese Journal of Nosocomiology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE This study was to investigate the carrier and infection of Clostridium difficile in clinic feces specimen,to analyze clinic characteristics,and to improve isolation rate and to provide basis on efficient prevention.METHODS C.difficile toxin A&B kit and anaerobic culture was conducted in 20 cases with diarrhea.Colonies suspected to be C.difficile,on the basis of their macroscopic appearance and characteristic odor,oxygen tolerance experiment,were confirmed by their biochemical characteristics(API 20A,bioMerieux).RESULTS After C.difficile selective culture,8 suspected colonies from 20 feces specimen were conducted by feces smear and oxygen tolerance experiment.6 of 8 was G+ rod bacteria with positive oxygen tolerance experiment.4 stains of C.difficile were identified by API 20A,positive rate was 20%;toxin detect was positive in 1 specimen(5%).CONCLUSIONS Infection of C.difficile Is associated with the basic disease.Watery feces specimen was prone to culture positive.
