Detection of Extended-spectrum ?-Lactamases in 5 Shigella sonnei Isolates
- VernacularTitle:产超广谱?-内酰胺酶宋内志贺菌的检测
- Author:
Ling ZHANG
;
Houzhao WANG
;
Wei LIANG
;
Wenli SU
;
Yong WANG
;
Dong JIN
;
Liuyu HUANG
- Publication Type:Journal Article
- Keywords:
Shigella sonnei;
Extended-spectrum ?-lactamases;
CTX-M-15
- From:
Chinese Journal of Nosocomiology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To detect ESBLs genes in 5 Shigella sonnei isolates.METHODS Susceptibility test to common antibiotics was performed through disk diffusion test,and ESBLs were confirmed according to CLSI.Conjugation experiment was performed to determine whether the resistance was transferable.The ESBLs gene was detected by PCR using universal primers for TEM,SHV,CTX-M,and the PCR products were also directly sequenced and analyzed.At the same time,the five isolates were analyzed by PFGE. RESULTS The five S.sonnei isolates were ESBLs producers and produced CTX-M-15 ESBLs,which were resistant to the most of the ?-lactams such as aztreonam,the first and second generation cephalosporins,cefotaxime,and non ?-lactam such as gentamicin,SF,but susceptible to ceftazidime,the 4th generation cephalosporins,Amikacin and meropenem.These strains were also intermediate to quinolones.CTX-M-15 gene could be transferred through conjugation.PFGE patterns of one isolate were different from the others.CONCLUSIONS Five S.sonnei isolates producing CTX-M-15 ESBLs are resistant to the most antibiotics.Clone spread is evident in these isolates.We should pay more attention to monitor these strains.
