Differentiation of human umbilical cord-derived mesenchymal stem cells into male germ cells
- VernacularTitle:体内诱导人脐带间充质干细胞向男性生殖细胞分化的实验研究
- Author:
Limin LIN
;
Xiaoying WU
;
Lian MA
- Publication Type:Journal Article
- Keywords:
Umbilical cord;
Mesenchymal stem cells;
Male germ cell;
Differentiate;
Male infertility
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the possibility of differentiation of human umbilical cord mesenchymal stem cells(HUCMSCs) into human male germ cells,and to explore a new source of cells for the treatement of male infertility.MethodsHUCMSCs were transplanted into the seminiferous tubules of the testis of infertile mice by microinjection.The survival rate,migration and germ cell markers of HUCMSCs in the mice testis were detected via immunohistochemistry,immunofluorescence and confocal laser scanning. ResultsHUCMSCs can survive in the mice testis for at least 120 days,and they can migrate from the lumens to the basement membrane.Immunofluorescence showed that HUCMSCs can further differentiate in the mice testicular environment,and express the germ cell marker.ConclusionsHUCMSCs can survive,migrate and differentiate into early male germ cell-like cells in the infertility mice testis after transplantation.
