Effect of NGX6 Gene with 5-Fu on the Apoptosis of Colon Cancer Cells
10.3969/j.issn.1000-8179.2009.21.012
- VernacularTitle:NGX6基因联用5-Fu对人结肠癌细胞凋亡的影响
- Author:
Ping LIAN
;
Qin GUO
;
Ya PENG
;
Zhiming XIAO
;
Fen LIU
;
Xiaoya WANG
;
Shourong SHEN
;
Guiyuan LI
- Publication Type:Journal Article
- Keywords:
Colon cancer;
NGX6 gene;
NF-κB;
Apoptosis
- From:
Chinese Journal of Clinical Oncology
2009;36(21):1239-1242,1247
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the effect of NGX6 with 5-Fu on the apoptosis of colon cancer cells. Methods: The NGX6-transfected HT-29 cell line with 5-Fu was used in the test group. HT-29 cell line with 5-Fu and PDTC was used in the control group. The expression of NF-κB was detected by EMSA. The proliferation of HT-29 cell line was assayed by MTT. The effect of NGX6 on the apoptosis was detected by FCM. HT-29 cells were double-stained by PI/Annexin-V and AO/EB and observed by fluorescence microscopy. Results: The expression of NF-κB was inhibited in NGX6 transfected colon carcinoma cell group and in colon carcino-ma cell group treated with PDTC. Treatment with the chemopreventive compounds 5-Fu and PDTC resulted in different responses in the effects of anti-proliferation and induced apoptosis of colon carcinoma cells. There was no significant difference in apoptosis between NGX6-transfected HT-29 call line with 5-Fu and the cells in the control group. NGX6 gene enhanced the effect of 5-Fu on the proliferation and apoptosis of colon carcino-ma cells. Conclusion: NGX6 gene can induce apoptosis and inhibit the proliferation of colon carcinoma cells. NGX6 gene can enhance the effect of 5-Fu on the proliferation and apoptosis of colon carcinoma cells through NF-κB pathway.
