Comparison of Three Methods on Relatedness Analysis of Acinetobacter baumannii Strains from Hospital Infection
- VernacularTitle:鲍氏不动杆菌医院感染株亲缘性分析3种方法学的比较
- Author:
Xiaomin XU
;
Lin CHEN
;
Zuhuang MI
;
Hui JIN
;
Shun ZHANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
PFGE;
AFLP;
Multiple gene cluster analysis
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the three methods on relatedness analysis of Acinetobacter baumannii strains in hospital infection.METHODS Twenty-seven A.baumannii strains caused hospital infection were analyzed by pulsed field gel electrophoresis(PFGE),amplified fragment length polymorphism(AFLP) and multiple gene cluster analysis.RESULTS PFGE analysis showed 19 strains isolated from clinic were with identical clone;AFLP analysis showed 19 strains isolated from clinic were with identical clone;but multiple gene cluster analysis showed 3 clones including 11 strains carried with 8 positive genes(TEM,OXA-23group,ADC,aac(3)-Ⅰ,aac(6′)-Ⅰ,ant(3″)-Ⅰ,qacE△1-sul1 and intⅠ1),6 strains with 7 positive genes(TEM,ADC,aac(3)-Ⅰ,aac(6′)-Ⅰ,ant(3″)-Ⅰ,qacE△1-sul1 and intⅠ1) and 4 strains with 6 positive genes(TEM,ADC,aac(3)-Ⅰ,ant(3″)-Ⅰ,qacE△1-sul1 and intⅠ1).CONCLUSIONS The resolving ability of multiple gene cluster analysis is higher than that of PFGE and AFLP in relatedness analysis of A.baumannii strains from hospital infection.
