Cloning,Expression and Sub-cellular Localization of APOBEC-3F and -3G and Their Effect on HBV
- VernacularTitle:人APOBEC-3F和-3G的克隆表达、亚细胞定位及其抑制HBV的研究
- Author:
Gefei WANG
;
Cheng PENG
;
Weizhong LI
;
Gang XIN
;
Yun SU
;
Youying CHEN
;
Guimei LIN
;
Kangsheng LI
- Publication Type:Journal Article
- Keywords:
APOBEC, expression, sub-cellular localization, HBV, nuclear location signal
- From:
Progress in Biochemistry and Biophysics
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
APOBEC(apolipoprotein B mRNA-editing enzyme catalytic-polypeptide) family members were reported as innate immune molecules with anti-viral activity for many viruses, such as HIV and HBV.In order to understand the function of APOBEC, the APOBEC-3F and-3G were cloned, expressed, and the sub-cellular localization of them was detected.The genes of APBEC-3F and-3G were cloned from PHA-stimulated PMBC and expressed in the MDCK cell by transfection.The sub-cellular localization of APOBEC-3F and-3G were detected by immunofluorescence.APOBEC-3F and-3G were cloned by RT-PCR and confirmed by DNA sequencing.The immunofluorescence indicated APOBEC-3F and-3G were located in the cytosal.APOBEC-3F and-3G could inhibit HBV replication effectively in HepG2.2.15 cell.APOBEC-3F and-3G could not be trans-located into nuclear by nuclear location signal(NLS) or bi-NLS(B-NLS).These results will help the future research on the function of APOBEC.
