Application of WCX magnetic bead for serum proteome profiling in cervical squamous cell carcinomas and its clinical significance
- VernacularTitle:WCX纳米磁珠在宫颈鳞癌血清蛋白质组中的应用及其临床意义
- Author:
Zhiguo ZHENG
;
Ting XIA
;
Yongzhe LI
;
Yun GAO
;
Hanzhou MOU
;
Shenhua XU
;
Yang XU
- Publication Type:Journal Article
- Keywords:
cervical cancer;
MALDI-TOF;
magnetic bead
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the serum proteomic pattern of the cervical cancer patients,to develope diagnostic model and to evaluate its clinical significance.Methods WCX magnetic bead and MALDI-TOF were used to detect the serum proteomic pattern of 77 patients with cervical squanmous cell carcinomas,13 patients with CINⅢ and 52 healthy women.Biomarker Wizard software was used to detect protein peaks and potential difference between cervical cancer and controls.The model was developed by Biomarker Patterns software.Results A diagnostic pattern consisting of three differential protein peaks was established with 100%(32/32)sensitivity and 93.8%(30/32)specificity.A sensitivity of 77.8%(35/45)and a specificity of 75%(15/20)in blind test were obtained.The diagnostic model also could discriminate CINⅢ and SCC-Ag negative patients from controls.Conclusion The diagnostic pattern combining 3974,3398,13732m/z protein peaks can discriminate not only cervical squamous cell cancer but also CINⅢ and SCC-Ag negative patients from controls.
