Construction and immune reaction of animal self-immunity discitis models
- VernacularTitle:自免疫椎间盘炎动物模型构建及其免疫反应
- Author:
Xinlong MA
;
Tao WANG
;
Jianxiong MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Discitis is an inflammatory lesion of the intervertebral disc.However,it attracts more attention on autoimmune factors.OBJECTIVE:To construct discitis animal model by destroying the fibrous ring and the cartilage lamina through the surgery,in addition,to explore the changes of autoimmune reaction.DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed at the Orthpeadic Laboratory of General Hospital of Tianjin Medical University from June to December 2007.MATERIALS:Fifty healthy,adult,New Zealand rabbits,irrespective of gender,were randomly divided into the model(n=30),sham operation(n=10)and control(n=10)groups.METHODS:Thirty rabbits were randomly selected after anesthetized,L3-4,L4-5,L5-6 intervertebral discs were exposed,discitis models were created by destroying the fibrous ring and the cartilage lamina with self-made transfixion pin.Animals in the sham operation group were exposed intervertebral discs without damage.Rabbits in the model and sham operation were executed to get the intervertebral disc specimens at weeks 1,2,4,8,and 12 after operation.MAIN OUTCOME MEASURES:①Hematoxylin-eosin staining,immunocytochemical comparison and lymphocytic expression.②The positive CD4 cells,CD8 positive T cells,as well as the expression of IgG,IgM were detected by immunohistochemistry and immunofluorescence technique.③Bacteriological cultivation.RESULTS:Under light microscope,the internal and external layer fibrous rings could be seen disordered,with inflammatory reaction and fibrosis in the model group.However,there was no same appearance in the sham operation and control group.Immunohistochemistry showed that there was more CD4 positive lymphocyte expressed at weeks 1 and 2 after operation,and the CD8 positive lymphocyte expressed more at 2 and 4 after operation.There was a few lymphocyte expressions in the sham operation group,and no expressed in the control group.Immunofluorescence demonstrated that immune globulin IgG,IgM was positive,a little expression was found in the model group at 1 week,which increased at the 2,4,and 8 weeks after operation.Only a little of IgG expression could be found in the sham operation and control groups.The bacteriological cultivation was negative.CONCLUSION:①Discitis animal model can be established by using the anterior external surgery to destroy the fibrous ring and the cartilage lamina.②After destroying the fibrous ring and the cartilage lamina,the contact between the autoantigen of the nucleus pulposus and blood circulation will provocate autoimmune reaction,which display in both cellular immunity and humoral immunity.③Autoimmune reaction plays a vital role in pathogenesy,pathological change and symptom generating of discitis.
