bla_(LAP-2),A Novel Subtype of ?-Lactamases Gene in Enterobacter cloacae in China
- VernacularTitle:阴沟肠杆菌中发现?-内酰胺酶基因新亚型bla_(LAP-2)
- Author:
Zhimi HUANG
;
Zuhuang MI
;
Qiuju CHU
;
Hao SHAN
;
Shouhui XIA
;
Weiping YANG
;
Haiyan YANG
;
Ling QIN
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
?-Lactamases;
Resistance genes;
Sequence analysis
- From:
Chinese Journal of Nosocomiology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the resistance genes in HZB9055 strain of Enterobacter cloacae isolated from the PLA 98th Hospital,Huzhou District,Zhejiang Province,China.METHODS HZB9055 Strain of E.cloacae was isolated from the inpatient in May,2004,41 kinds(or groups) of genes were analyzed by PCR and verified by DNA sequencing.RESULTS In HZB9055 strain of E.cloacae,3 kinds of genes were positive including blaLAP,blaMIR and qnrS.The 38 kinds of rest genes were all tested negative.The blaLAP gene sequence including 858 nucleotides,which had the amino acid mutation in position 193 compared with LAP-1 type narrow-spectrum ?-lactamase(GenBank accession number:EF026092),and was nominated LAP-2(GenBank accession number:EU159120).CONCLUSIONS At least 3 kinds of resistance genes exist in HZB9055 strain of E.cloacae,and blaLAP-2 is a novel subtype of ?-lactamases gene.
