Adenosine A_1 Receptor Agonist Prevents Angiotensin Ⅱ Induced Hypertrophy in Cultured Myocardial Cells of Neonatal Rats
- VernacularTitle:腺苷A_1受体激动剂抑制血管紧张素Ⅱ诱导的心肌细胞肥大
- Author:
Jie LIU
;
Yuhong YANG
;
Hongxin WANG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Cardiac hypertrophy;
R(-)-N6-(2-phenylisopropy 1)adenosine;
Angiotensin Ⅱ;
CaN
- From:
Chinese Journal of Hypertension
2007;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate the inhibitory effect of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropy1)adenosine(R-PIA)and cross-talk between adenosine A1 receptor and CaN in cultured myocardial cells hypertrophy induced by Ang Ⅱ.Methods The protein synthetic rate was determined by measuring the incorporation of [3H]-leucine into myocyte protein;the cardiomyocytes volumes were measured by computer photograph analysis system;the expression of atrial natriuretic polypeptide(ANP)and CaN were determined by Western-blot.Results R-PIA(1 ?mol/L)inhibited Ang Ⅱ(0.1 ?mol/L)-induced increase of [3H]-leucine incorporation [R-PIA:(976.2?89.0)vs Ang Ⅱ:(1130.7?102.7)count per min per well,P
